Establishment of LC-MS methods for the analysis of palmitoylated surfactant proteins[S]

Establishment of LC-MS methods for the analysis of palmitoylated surfactant proteins[S]

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The surfactant proteins (SPs), SP-B and SP-C, are important components of pulmonary surfactant involved in the reduction of alveolar surface tension.Quantification of SP-B and Arm and Leg Protection SP-C in surfactant drugs is informative for their quality control and the evaluation of their biological activity.Western blot analysis enabled the quantification of SP-B, but not SP-C, in surfactant drugs.

Here, we report a new procedure involving chemical treatments and LC-MS to analyze SP-C peptides.The procedure enabled qualitative analysis of SP-C from different species with discrimination of the palmitoylation status and the artificial modifications that occur during handling and/or storage.In addition, the method can be used to estimate the total amount of SP-C in pulmonary surfactant drugs.

The strategy described here might serve as a prototype to establish analytical methods Disinfectant for peptides that are extremely hydrophobic and behave like lipids.The new method provides an easy measurement of SP-C from various biological samples, which will help the characterization of various experimental animal models and the quality control of surfactant drugs, as well as diagnostics of human samples.